# TOPIC: Microscope AIM: How is a specimen viewed with a ...

Topic: Microscope Aim: How can we measure a cell using a microscope? Do Now: 1. Copy the aim onto your notes sheet. 2. Complete Microscope Check pink sheet 3. Take out a piece of loose-leaf for todays notes. HW: Cells and Cell Processes Exam Review Sheet

(BOTH SIDES) 1. Where should the microscope be safely placed? On a tabletop or other flat surface. 2. Why is the lowest power objective used first?

It provides a larger field of view. LOW HIG H Ruler observed

under low power LARGER FIELD OF VIEW Ruler observed under high power SMALLER FIELD OF VIEW

3. Identify the objective that gives a larger field of view. Low power LOW HIG

H 4. Identify what occurs to the field of view when switching from low to high power. The field of view decreases. The amount of light decreases.

5. Identify the part that used to focus the slide when using the 4x and 10x objectives. Coarse adjustment 6. Identify the part that used to focus the slide when using the 40x objective. Fine adjustment

The diagram represents a cell in the field of view of a compound light microscope. In which direction should the slide be moved on the microscope stage to center the cell in the field of view?

1. toward A 2. toward B 3. toward C 4. toward D Units to measure cells Micrometers/Microns m

1mm = 1000m Cells are so small that even a cluster of these cells from a mouse only

measures 50 microns. The human ovum is typically around between 120 to 150

micrometers which is the largest cell in the human body. It is visible to the human eye under a microscope.

The head of the sperm is on average 5 to 6 micrometers. The flagellum is up to 50 micrometers long.

Paper thickness = about 150 m Human hair diameter = 80-100 m SHRIMP EGGS

mm um Move decimal three places to the RIGHT 3.2 mm = ? m 3. 20 0 3200 m Are there SI units smaller than micro????

"nano-" = 1/1,000,000,000 (billionth) "pico-" = 1/1,000,000,000,000 (millionth of a millionth, "micro micro-") 0.7634mm = ? m 0.7 6 3 4 763.4 m

um mm Move decimal three places to the LEFT 789 m = ? mm 7 8 9. .789 mm 53.6 m = ? mm

0 5 3. 6 .0536 mm 1. 1m m

3.2mm Field of view = = 3200 m 2. 500u

m 1mm 1000u = m 3. 1000u

m 2000/2 = 1000 2 mm = 2000um 4.

500u m 250u m 1000um

5. 1000u m 500u m

2000um 6. 500u m

250u m 125u m 1000um

7. 2500u m 1250u m

625u m 5mm 5000u 8.

1000u m 5m m =

5000um 9. 1000u m 7000um

10. 400um 2000um Lets summarize:

1. Identify the units used to measure cells. 2. Which way do you move the decimal when converting from mm to um? 3. Which way do you move the decimal when converting from um to mm? 4. Describe the two procedures that must be followed before switching to high power. 5. Explain what happens to the amount of

light in your field of view when switching from low to high power. Determine the length of each cell in um. 500um Diameter = 1mm

1000um = 500um 1000u m 500um

Diameter = 2000um = 500um Determine the length of each cell in um. 500um 1000um

Diameter = 1000um 1000/6 = 166.7um Diameter = 2mm = 2000um

1. Which way should 700um 1400um you move the slide to center the cell in the 350umfield of view?

Move the slide to the right (towards B) 2. Determine the approximate length of the cell. a. b. c.

d. 300um 500um 700um 1000um

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