Activity 6 Cellular Respiration Activity 9 Pitfall Traps

Activity 6  Cellular Respiration Activity 9  Pitfall Traps

Activity 6 Cellular Respiration Activity 9 Pitfall Traps Writing a Lab Report Week of October 1st 2018 Ver 1.9. Last update 10/5/2018 4:20:25 PM Field Trip Huntley Meadows Park th Saturday October 6 9 a.m. 3701 Lockheed Blvd Alexandria VA https:// Molecules and Processes of Life Activity 6 Cellular Respiration and the Effect of Pollutants on its Rate Cellular respiration Process by which energy in food molecules is converted into a form the cell can use (ATP). C6H12O6 + 6O2 6CO2 + 6H2O (+ ATPs) Part A Effect of Carbohydrates Prepare 2 tubes: One with a carbohydrate source. One without a carbohydrate source. Cellular respiration rate is expressed by gas volume

produced. WE ARE USING DIFFERENT TUBES THAN THOSE DESCRIBED IN THE LAB MANUAL. NOTE: The fermentation tubes cost ~$30 each and are fragile. Please exercise extreme caution when INSTEAD of the setup shown in Fig. 6.1, we are using fermentation tubes, which look like this: Manipulate tubes to minimize gas in the closed end of the tube, so that it looks like this: Set up fermentation tubes: Tube 1: Mix 1.25 mL yeast + 13 mL warm water in 25 mL beaker and then pour into tube #1. Tube 2: Thoroughly mix 1.25 mL yeast + 1.25 mL sugar in 25mL beaker, then add 13 mL warm water to Over time, as cellular respiration proceeds

and produces the product CO2 gas, it will accumulate in the closed tip of the tube. You can measure the volume of the gas using the graduations on the tube. Table 6.1. Gas (CO22) volume (mL) at 1 minute intervals for 10 minutes for centrifuge tube containing yeast and water versus centrifuge tube containing yeast, water and sugar Tube # Contents 1 Yeast+Water Yeast+Sugar+W 2 ater Gas Volume (mL) in Centrifuge Tube at Time (min) 0 1 2 3 4 5 6 7 8 9 10 Record individual group data for volume of gas

present in each tube (w/ and w/o sugar) at one minute intervals for period of 10 minutes. Part B Effect of Pollutant Repeat the process with a third fermentation tube with an added pollutant (1.25 mL). The cellular respiration rate will be expressed by the volume of gas produced. Assigned Pollutants Group Isopropyl alcohol 1: Group Vinegar 2: Group Salt solution, 10% 3: Group Baking soda solution, 4: 5% Group Bleach solution, 1% 5: Group Soap solution, 10% 6: Data to Record Write down volume of gas present in tube with

pollutant at 1 min intervals for period of 10 min in Table 6.2. Grou Gas Volume (mL) in Centrifuge Tube at p # Simulated Pollutant 1 2 Isopropyl alcohol Vinegar Salt solution, 10% Baking soda soln., 5% Bleach solution, 1% 3 4 5 0 1 2 3 Time (min)

4 5 6 7 8 9 10 Table 6.2. Gas (CO22) volume (mL) at 1 minute intervals for 10 minutes for 6 centrifuge tubes, each containing yeast, water, sugar, and one of six simulated pollutants Data to Record Record individual and group data for cellular respiration rate (gas production in mL/min). Tube Contents Yeast + water Yeast + water + sugar Yeast + water + sugar + isopropyl alcohol Yeast + water + sugar + vinegar Yeast + water + sugar + salt solution, 10% Yeast + water + sugar + baking soda soln, 5% Yeast + water + sugar + bleach Total Volume

of CO2 Produced Cellular Respiration Rate (mL/min) Table 6.3. Total volume of CO22 produced and cellular respiration rate (mL/min) for 8 combinations of yeast, water, sugar, and simulated pollutant Diversity of Life Activity 9 Pitfall Traps Retrieve traps. If trap contained LIVE organisms, place bag(s) into freezer for ~5 minutes. Place into petri dish, observe with dissecting microscope and count and identify organisms. Record data in Table 9.1. If your trap contained no arthropods or was filled with water, use two containers each containing a collection of photos representing arthropods caught in a trap. Count and identify organisms,

recording data in Table 9.1. How to identify organisms Identify organisms by type: i.e. bee, ant, beetle, fly, spider, etc. If multiple distinct types, e.g. two spider types, use spider 1 and spider 2. If it cant be identified, call it unknown. Count number of organisms (=absolute abundance) of each type. Calculate relative abundance of each organism (ni/ Table 9.1. Organism type, absolute abundance and relative abundance for organisms in the two pitfall traps in group quadrat Pitfall Trap #1 Pitfall Trap #2 Organism Absolute Relative Organism Absolute Relative Type Abundance Abundance Type Abundanc Abundanc ("Species") (ni) (pi) ("Species") (i) e (ni) e (pi) (i)

ant 2 2/13=0.15 bee 1 1/13=0.08 spider 3 3/13=0.23 beetle 5 5/13=0.39 spider 2 2 2/13=0.15 i= 5 N = 13 pi = 1.00 i = N= pi = 1.00 Ds = 1 ((nni(ni-1))/(N(N1))) Calculate Simpsons Diversity Index. For previous example data it would be: 1 ((2(2-1))+(1(1-1))+(3(3-1))+(5(5-1))+(2(2- 1))) / (13(13-1)). e 9.2. Simpsons Diversity Index for two pitfall trap samples per lab g Group #: 1 Pitfall Trap # 1 2

Simpson's Diversity Index: 2 3 4 5 6 1 2 1 2 1 2 1 2 1 2 Writing a Lab Report Formal Lab Report Project Timeline Date: 9/30/18 10/01/18 10/5/18 10/22/18 10/26/18 11/5/18 11/09/18 Project Component Completion of Scientific Paper On-Line Tutorial. Pre-lab lecture on writing a lab report. Lab activity (constitutes basis of lab report). Peer-review of lab reports. Report due (submit to BlackBoard) by beginning of lab. Writing a Lab Report

Goal: introduce writing style used to present the process and results of scientific experimentation. 1. Read activities in lab manual structured similar to 2. 3. 4. 5. 6. scientific papers. Complete Scientific Paper Tutorial On-line Assignment. Review expected content and format. Read Writing a Lab Report section in lab manual. Read grading rubric (will be available on Blackboard). Participate in peer-review process to evaluate lab reports (you will be graded on participating in this process, but your peers evaluation will not affect your final grade.) Writing a Lab Report Data and observations will be made available, but the formal lab report must be the independent work of each student. You are required to submit an electronic copy of your report. That copy will be checked against a database for plagiarism.If you have any doubt what constitutes plagiarism, please ask. Honor Code Do not copy each other's graphs, figures, tables, illustrations or any written portion of the formal lab report.

Anyone who engages in this activity will be considered to be in violation of the Honor Code and will be subject to its process and consequences. Please visit the GMU Office of Academic Integrity website at to make sure that you understand the Honor Code and Week of October 22nd: Peer Review During lab, you will review two of your classmates reports. Purpose of peer-review process is to provide you with the opportunities to: Evaluate and understand the formal lab report grading rubric prior to being graded; See examples of different lab reports; Give feedback to improve lab reports. Peer-reviewed evaluations will not be part of lab Participation in peer-review process is report grade. however worth 12 points (4%) of lab

Week of November 5 : Lab reports due BEFORE lab! th LATE SUBMISSIONS WILL NOT ACCEPTED. MUST be submitted via BlackBoard e-mailed reports not accepted. Must by in .doc or .docx format. Grading policy: Lab report will be graded strictly! See grading rubric on BlackBoard. Week of December 3 : Lab Reports Graded rd Peer-review participation = 12 points (4%) of lab grade. Peer-review report grades = will not count. Formal lab report grade = 57.25 points (19.08%) of lab grade. Essential Parts of a Scientific paper Title Describe core contents of paper. Abstract Summarize major elements of paper. Introduction Provide context and rationale for study. Methods Describe experimental design and procedures so

anyone could reproduce your experiment. Results Summarize findings, without interpretation. Discussion Interpret findings of study. Literature Cited List all scientific papers, books and websites you used. Title 1 = Title is present and all of the following conditions met 1) title is thoroughly descriptive of the experiment (for example, Effect of Rain Quantity and Frequency on Oak Tree Seedling Growth), 2) title located on a title page, 3) title page in correct format. Example 0.5 = Title is present 0.75 = Title is present 0.25 = Title is present but not thoroughly and thoroughly but not thoroughly descriptive of descriptive of descriptive of experiment (for

experiment (for experiment (for example, Effect of example, Effect of example, Effect of Rain on Tree Rain on Tree Growth) Rain on Tree Growth) Growth) and at least 0 = No title present. and at least of the and both of the of the following following conditions following conditions conditions are are present: 1) title are present: 1) title present: 1) title not not on a title page, 2) not on a title page, 2) on a title page, 2) title page not in title page not in title page not in correct format. correct format. correct format. Be descriptive, but concise: Aerially Applied Diflubenzuron on Decomposition Rate and Litter Arthropods in Prince William County, Virginia NOT

Report or on Bugs in Leaves, Formal Lab Report for EVPP 110 Abstract 0= No main 0.25 = Summarizes concepts main concepts of summarized or some sections of abstract not the report. present. 0.25 = Information 0.5 = Information is is presented in an presented in the 0= Information order that is same order as the order is irrelevant different from the sections of the since abstract not order in which the report are supposed present. report sections are to be arranged. supposed to occur. 0.25 = Length of 0.35 = Length of

0.5 = Length of abstract is >1 abstract is < 1 abstract is < 1 page, page, singlepage, doublesingle-spaced. spaced or doublespaced. spaced. 1.5 = Quality of 1.25 = Quality of 1.0 = Quality of abstract would be abstract would be abstract would be described as described as above described as 0.5 = Summarizes main concepts of every section of the report. 0= Length is irrelevant since abstract not present. 0.75 = Quality of 0= Quality absent abstract would be since abstract not described as below present. 8 = Provides a general review of peer-reviewed literature relevant to the experiment, addressing all five

of the questions. 6 = Provides a general review of peer-reviewed literature relevant to the experiment, addressing four of the five questions. 4.5 = Provides a general review of peer-reviewed literature relevant to the experiment, addressing three of the five questions. 1.25 = Contains 1.0 = Contains three or more intwo in-text text literature literature citations but they citations from are either not from peer-reviewed peer-reviewed sources, in APA sources OR are format. not in APA format. 0.5 = Describes 1.5 = Describes

1.0 = Describes main objectives main objectives of main objectives of of experiment experiment experiment incompletely and thoroughly and in a incompletely but in a non-logical logical order. in a logical order. order. 1.5 = One or 3.0 = All 2.25 = All more hypotheses hypotheses stated hypotheses stated not included but in the proper but incorrect those included format. format used. were in proper format. 0.75 = 0 = No 1.5 = Experimental Experimental experimental design briefly design explanation design 1.5 = Contains three or more intext literature citations from peer-reviewed sources, in APA format.

3.0 = Provides a general review of peer-reviewed literature relevant to the experiment, addressing two of the five questions. 0.75 = Contains two in-text literature citations but they are either not from peerreviewed sources OR are not in APA format. 1.5 = Provides a general review of peer-reviewed 0 = No review of literature peer-reviewed relevant to the literature experiment, included. addressing one of the five questions. 0.25 = Contains 0.5 = Contains one in-text one in-text literature citation 0 = No in-text literature citation but it is either not

literature citations from a peerfrom a peerincluded. reviewed source, reviewed source in APA format. OR is not in APA format. Introductio n 0 = Main objectives of experiment not described. 0.75 = One or more hypotheses not included and those included were in incorrect format. 0 = No hypotheses stated. 6 = Concise, easy- 4.2 = Concise, easy- 3.0 = Description 1.8 = Description of 0 = No description to-follow description to-follow of materials and materials and of materials and of materials and description of procedures was procedures was procedures procedures provided materials and

either not concise either not concise provided. in enough detail for procedures and easy to follow and easy to follow experiment to be provided in enough OR lacked OR lacked sufficient repeated, ALL in detail for sufficient detail for detail for paragraph form. experiment to be experiment to be experiment to be repeated, but all or repeated, in repeated, but all or some was provided paragraph form. some was in in bulleted or bulleted or numbered format. numbered format. 2 = Specifically 1 = Did not 0 = No description described what data completely describe of the data that would be collected the data that would would be collected and when in the be collected OR did or when it would experiment it would not adequately be collected was collected. describe when in provided. the experiment it would be collected. 2 = Describes how 1 = Describes how 0 = No description all data will be some data will be of how data will be

analyzed. analyzed. analyzed provided. 3.0 = Quality of 2.25 = Quality of 1.5 = Quality of 0.75 = Quality of 0 = Quality absent introduction introduction introduction introduction since methods Methods 6 = Describes all main 5 = Describes at least 4 = Describes all 3 = Describes < of 2 = Describes < 0 = Main findings of findings of experiment of main findings of main findings of main findings of of main findings of experiment were not and cites all key experiment and cites experiment and experiment and cites experiment and cites described and no key pieces of data, in at least of key cites all key pieces < of key pieces of < of the key pieces pieces of data were paragraph form pieces of data, in of data, in nondata, in paragraph of data, in noncited paragraph form paragraph form form paragraph form

3 = All data 2.4 = All data 1.8 = All data 1.2 = Part of data 0.6 = Part of data 0 = No data summarized in at least summarized in at summarized in at summarized in at summarized in at summarized in a table one table/figure (T/F), least one table or least one table or least one table or least one table or or figure multiples arranged figure (T/F), multiples figure (T/F), and no figure (T/F), multiples figure (T/F), and logically and arranged logically more than two of are arranged more than two of numbered and numbered following conditions logically and following conditions sequentially by type, sequentially by type, present: not numbered present: not section does not begin section does not arranged logically, sequentially by type, arranged logically, with T/F, all T/F are begin with T/F, all T/ not numbered section does not begin not numbered referenced by their

F are referenced in sequentially by type, with T/F, all T/F are sequentially by type, number within paragraph portion of section began with referenced by their section began with paragraph portion of section but not by T/F, T/F not number within T/F, T/F not section their T/F number referenced at all in paragraph portion of referenced at all in paragraph portion of section paragraph portion of section section 4 = Table/figure 3.6 = Table/figure 2.8 = Table/figure 2 = Table/figure 1.2 = Table/figure 0.5 = Table/figure 0 = Table/ figure format: Each table has format: No more than format: More than format: More than format: More than format: More than format: No data above it a descriptive one of the following one but no more three no more than five but not more seven of following summarized in a table title that begins with conditions present: than three of the five of the following than seven of conditions present:

or figure Table followed by its title absent; title not following conditions conditions present: following conditions title absent; title not number; each figure descriptive; title in present: title absent; title absent; title not present: title absent; descriptive; title in has below it a wrong place; title title not descriptive; descriptive; title in title not descriptive; wrong place; title descriptive title that doesnt begin with title in wrong place; wrong place; title title in wrong place; doesnt begin with begins with Figure Table..; or title doesnt begin doesnt begin with title doesnt begin Table..; or followed by its Figure; units with Table..; or Table..; or with Table..; or Figure; units number; tables absent in tables; axis Figure; units Figure; units Figure; units absent in tables; axis contain units in their labels missing, axis absent in tables; absent in tables; axis absent in tables; labels missing, axis row/column headings units missing, axis axis labels missing, labels missing, axis axis labels missing, units missing, axis as appropriate; figures values missing

axis units missing, units missing, axis axis units missing, values missing have on both axes axis values missing values missing axis values missing labels, units and values Results 3 = All hypotheses 2.25 = All re-stated in the hypotheses reproper format stated but incorrect format used 3 = No new results were introduced here and no incidences of the reiteration of results being presented as interpretation of results 9 =All data interpreted (in same order in which results were presented), by explaining how findings link to basic scientific concepts of the experiment and how they link to

findings of other researchers 1.5 = One or 0.75 = One or 0 = No more hypotheses more hypotheses hypotheses not re-stated but not re-stated and re-stated those re-stated those re-stated were in proper were in incorrect format format 2.5 = No new 1.5 = Two new 0.75 = >1<3 new 0 = >3 new results results results introduced results introduced here introduced here here and/or >1<3 introduced here but at least one and/or two incidences of the and/or >3 incident of the incidences of the reiteration of incidences of reiteration of reiteration of results being reiteration of results being results being presented as results being presented as presented as interpretation of presented as interpretation of interpretation of results interpretation of

results results results 7.5 =At least of 6.5 =At least 5.5 =At least of 4.5 = <> of 3.0 = <> of data interpreted of data data interpreted data interpreted data interpreted (in same order in interpreted and and more than an at least one of an more than one which results at least one of one of the the following of the following were presented), the following following conditions were conditions were by explaining how conditions were conditions were present: present: findings link to present: present: interpretation interpretation basic scientific interpretation interpretation was not in same was not in same concepts of the was not in same was not in same order in which order in which experiment and order in which order in which results were results were how they link to results were results were presented; no presented; no

findings of other presented; no presented; no explanation of explanation of researchers explanation of explanation of how findings link how findings link how findings link how findings link to basic to basic scientific to basic to basic scientific scientific concepts of the scientific concepts of the concepts of the experiment; no concepts of the experiment; no experiment; no explanation of experiment; no explanation of explanation of how findings link Discussio n 1.5 = < of data 0 = No data interpreted and interpreted in one or more of context of the following explaining how conditions were findings relate present: to scientific interpretation was concepts of the not in same order experiment

in which results and/or how were presented; findings relate no explanation of to those of how findings link other to basic scientific researchers concepts of the experiment; no explanation of how findings link to those of other 5 = Addresses other 3.75 = Addresses 1.75 = Addresses issues, including all other issues, other issues, of the following: including 2 of the including 1 of the sources of following 3: sources following 3: uncertainty that may of uncertainty that sources of have led to unclear may have led to uncertainty that results; further unclear results; may have led to investigations that further unclear results; could/should be investigations that further done to gather more could/should be investigations that information; done to gather more could/should be suggestions for information;

done to gather improving current suggestions for more information; experiment; improving current suggestions for alternative experiment; improving current explanation for alternative experiment; positive or negative explanation for alternative results. positive or negative explanation for result. positive or negative result. 1.5 = States 1 = States whether 0.75 = States whether or not the or not the data whether or not the data supports all supports all data supports hypotheses and why/ hypotheses, but some hypotheses how. does not address and why/how. why/how. 1.5 = All conclusions 1 = All conclusions 0.75 = Some that could be drawn that could be drawn conclusions that from results were from results were

could be drawn clearly stated. stated, but not from results 0.75 = Addresses 0 = Did not other issues, but address other none of the issues at all. following were included: sources of uncertainty that may have led to unclear results; further investigations that could/should be done to gather more information; suggestions for improving current experiment; alternative explanation for positive or negative result. 0.5 = States 0 = Does not whether or not the address whether data supports some or not the data hypotheses, but supports the does not address hypotheses or why/

why/how. how. 0.5 = Some 0 = No conclusions conclusions that were stated. could be drawn from results were stated, Discussio n Literature Cited 1.5 = Lists 1.25 = Lists 1.0 = Lists 0.75 = Lists 0.5 = Lists 0.25 = Lists 0 = No at least at least two sources two sources one source one source sources three three (not (not (not (not listed. sources (not sources (not including including including including including lab including lab manual) lab manual) lab manual) lab manual) manual) in lab manual) in APA but not in in APA but not in APA format. but not in

format. APA format. format. APA format. APA format. 1.5 =All 1.0 = Only 0.5 = Only 0 = No inlisted two of listed one of text sources are sources are listed citations of cited in body cited in sources listed of report in body of cited in sources and/ proper report in body of or all in-test format and proper report in citations all in-text format and/ proper absent form citations are or one informat and/ list. listed. text citation or two innot included text General 3 = Writing style: All of the following conditions met; written in scientific style; correct tense (past) and person (not first) used

throughout. 2 = Writing style: One of the following conditions not met; written in scientific style; correct tense (past) and person (not 1st) used throughout. 1 = Writing style: Two of the following conditions not met; written in scientific style; correct tense (past) and person (not 1st) used throughout. 1 = All required sections are 0.75 =All required sections are 0.35 = At least one required present and each has the present and no more than one section is missing, or two or appropriate section heading. appropriate section heading more appropriate section missing. headings missing. 3 = Sentence structure: 2 = Sentence structure: Most 1 = Sentence structure: Less Sentences 1) are extremely well sentences 1) are extremely well than of sentences 1) are developed sentences, 2) express developed sentences, 2) express extremely well developed ideas clearly, 3) are concise, and ideas clearly, 3) are concise, and sentences, 2) express ideas 4) flow well. 4) flow well. clearly, 3) are concise, and 4) flow well. 0 = Writing style: None of the

following conditions not met; written in scientific style; correct tense (past) and person (not 1st) used throughout. 0 = Two or more required sections are missing, or three or more appropriate section headings missing. 0 = Sentence structure: Sentences dont express ideas well and are poorly developed; sentence structure is sometimes so poor that it makes reading and understanding difficult; sentences would sound strange if read out loud. 4 = Grammar, punctuation, 2.5 = Grammar, punctuation, 1 = Grammar, punctuation, 0 = Grammar, punctuation, spelling: Writing is nearly error spelling: Writing contains <3 spelling: Writing contains >3 spelling: Writing contains >5 free. No more than two insignificant errors (doesnt <5 insignificant errors insignificant errors (doesnt insignificant errors (doesnt interfere with comprehension or (doesnt interfere with interfere with comprehension or interfere with comprehension or distract from message) per page comprehension or distract distract from message) per page, distract from message) in entire and no more than one from message) per page, and/ and/or more than two significant paper and no significant errors significant errors that interfere or more than one significant errors that interfere with that interfere with

with comprehension or distracts error that interferes with comprehension or distracts from comprehension or distracts from from message. comprehension or distracts message. message. from message. 2 = Format: All of the following 1.25 = Format: Incorrect font 0.5 = Format: typed in Times 0 = Format: Incorrect font style Optional: Guides to Writing Scientific Articles shop.pdf Whats Due Activity 6 Activity 9 Weekly Data Sheet pages 115 179 Weekly Write-Up pages 119-120 183-184 PowerPoint available at: -110

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