Ex. 3: Staining Techniques - Gram Stain, part 2 (Live Cultures)

Ex. 3: Staining Techniques - Gram Stain, part 2 (Live Cultures)

Ex. 3: Staining Techniques - Gram Stain, part 2 (Live Cultures) SLO: Apply previously learned technique to life cultures

How to label a slide Circles on back

Other info on front Little Finger Technique

Smear Preparation From Solid Medium

From Liquid Medium Suspend bacterial sediment in culture tube

by gently tapping on it. Transfer one loopful of the bacterial suspension to slide and spread out (You

may exceed the limits of the circle) Air-dry and heat-fix

Transfer one loopful of H2O into circle area of slide. Using loop, suspend very small quantity of colony of interest in

that drop of water and spread the suspension evenly. It should only appear slightly milky. Do NOT use too many

bacteria Air-dry and heat-fix Gram Stain Review

cheek cell in the center covered by various microorganisms present in dental plaque

Artifacts Precipitated crystal violet stain may resemble cocci. Clues: dense clumping, small size of the gram-positive dots. Paucity of organisms elsewhere in the field.

Examples of Gram Stains Gram Stain of Urine

Gram Stain of Urine

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